Genome organization of <Emphasis Type="Italic">Treponema zioleckii</Emphasis> |
| |
Authors: | L Sikorová M Piknová P Javorský P Prista? |
| |
Institution: | Institute of Animal Physiology, Slovak Academy of Sciences, 040 01, Kosice, Slovakia, luciannaa@pobox.sk. |
| |
Abstract: | Genome analysis of Treponema zioleckii proved that, in this bacterium, besides chromosomal DNA, a relatively small extrachromosomal DNA element is present. This element was shown to be a double-stranded circular plasmid DNA of approximately 7 kbp; it was designated as pKT. The plasmid was characterized by molecular and bioinformatic analysis. No pKT homologous DNA sequences were detected in other rumen Treponema strains. The overall G+C content of the pKT plasmid is approximately 56 %, which is higher than in other Treponema plasmids or genomes. The Rep module of the pKT plasmid consisting of the rep gene and the region of repeats was identified within a 1.6-kbp fragment. The putative rep gene encodes the replication protein belonging to the pfam04796 RepA_C family of proteins with the highest similarity (25 % within 249 amino acids) to the RepA protein from the green sulfur bacterium Prosthecochloris aestuarii. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|