Calmodulin activates adenylate cyclase from rabbit heart plasma membranes |
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| Authors: | M P Panchenko V A Tkachuk |
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| Affiliation: | Institute of Experimental Cardiology, Cardiology Research Center of the USSR, Academy of Medical Sciences, 3rd Cherepkovskaya street 15A, Moscow 121552, USSR |
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| Abstract: | It was shown that calmodulin (CM) activates the adenylate cyclase (AC) of rabbit heart light sarcolemma in the presence of micromolar free Ca2+ concentrations and this effect is blocked by trifluoroperazine and troponin I. GTP (in the presence of isoproterenol) and Gpp(NH)p are able to increase the CM-dependent activity of enzyme. It was concluded that there is no special CM-dependent "form' of AC in the heart and the common catalytic component of AC can be regulated both by CM and guanine nucleotide-binding regulatory component (N-protein). In the presence of Ca2+ and guanine nucleotide heart AC exists as a complex: CM-catalytic component-N-protein. |
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| Keywords: | Calmodulin Adenylate cyclase Cardiac light sarcolemma Guanine nucleotide Gpp(NH)p guanosine 5'-(β,γ-imido)triphosphate |
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