Bismuth chemistry and the glutaraldehyde insensitive staining reaction

The free metal ion, Bi3+, is the only chemical species of bismuth that stains a strongly bismuth-reactive molecule, polyarginine, in vitro. The bismuth solution specifically requires tartrate as a chelating agent for the reaction to occur between pH 7.4 and 8.0. Since Bi3+ reacts strongly with polyarginine, creatine and ATP fixed to cellulose acetate strips and DEAE-cellulose and P-cellulose, the free metal ion (Bi3+) may bind to phosphate or guanidyl groups, or both, after glutaraldehyde fixation.