Characterization of Thermophilic Proteolytic Spore-Forming Bacteria from a Geothermal Site in Lithuania Based on 16S rDNA RFLP and ITS-PCR Analyses1 |
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| Authors: | Kuisiene N Jomantiene R Valiunas D Chitavichius D |
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| Institution: | (1) Department of Plant Physiology and Microbiology, Faculty of Natural Sciences, Vilnius University, Chiurlionio 21/27, Vilnius, 2009, Lithuania;(2) Institute of Botany, Academy of Sciences of Lithuania, Zaliuju ezeru 49, Vilnius, 2021, Lithuania |
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| Abstract: | Forty-two strains of gram-positive, aerobic, heterotrophic, obligately thermophilic, spore-forming bacteria were isolated from a geothermal site near the Baltic Sea in Lithuania. All of the strains were able to hydrolyze collagen and/or casein. Since characteristics of proteolytic activity are correlated with taxonomic positions of bacteria, the strains were grouped on the basis of molecular biological analyses. On the basis of RFLP patterns of 16S rDNA and 16S–23S rDNA ITS-PCR analysis, the strains were subdivided into nine groups. |
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| Keywords: | thermophilic 16S rRNA gene 16S– 23S rDNA intergenic spacer region RFLP analysis |
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