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Saccharomyces cerevisiae 2-mum DNA. An analysis of the monomer and its multimers by electron microscopy.
Authors:Hans-Dieter Royer and Cornelis P. Hollenberg
Affiliation:(1) Abt. Beermann, Max-Planck-Institut für Biologie, Spemannstraße 34, D-7400 Tübingen, Federal Republic of Germany
Abstract:Summary The non-tandem inverted duplication in the 2-mgrm DNA of Saccharomyces cerevisiae has a length of 0.19 mgrm and is located asymmetrically along the molecule. The majority of the dumb-bell structures that are formed upon denaturation and selfannealing of the 2-mgrm monomer consists of the renatured inverted duplication sequences as double stranded stem and two single stranded loops of 0.67 mgrm±0.06 mgrm (S-loop) and 0.86 mgrm±0.05 mgrm (L-loop) length. Two additional size classes which comprised 5–10% of the measured molecules had contour lengths of around 1.7 mgrm and 2.1 mgrm. The smaller dumb-bells contained two S-loops and the larger dumb-bells contained two L-loops as was shown by heteroduplex mapping with an HindIII fragment from the L-loop. Two models which assume illegitimate or site specific recombination, are presented to explain the generation of double S-loop and double L-loop molecules. At least part of the 4-mgrm and 6-mgr circular molecules present in the yeast supercoiled DNA fraction are shown to be dimers and trimers of 2-mgrm monomers, but often with inverted loop segments most probably due to intramolecular recombination between sequences of the inverted duplication.2-mgrm DNA is used to indicate the supercoiled DNA fraction although in our measurements the average monomeric length is 1.9 mgrmPart of this work has been presented at the Conference: The Genetics and Biogenesis of Chloroplasts and Mitochondria, Munich, August, 1976
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