The effect of varying 3H-shiperone concentration on its binding parameters |
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Authors: | Elizabeth J. Hartley Philip Seeman |
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Affiliation: | Department of Pharmacology University of Toronto Toronto, Canada M5S 1A8 |
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Abstract: | Higher concentrations of spiperone were required to compete against the binding of 3H-spiperone to calf caudate when higher concentrations of 3H-spiperone were used. The Cheng-Prusoff equation did not adequately apply to this hydrophobic ligand, since the Ki values varied directly with the 3H-spiperone concentration. The equation was only adequate and gave a constant Ki when the total concentrations of 3H-spiperone were replaced by the measured free concentrations. It is suggested that IC50 values are best reported as such, unless the free concentrations are actually determined directly. |
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