Immunosuppressive effect on T cell activation by interleukin- 16-cDNA-transfected human squamous cell line |
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Authors: | Fujita T Matsumoto Y Hirai I Ezoe K Saito T Yagihashi A Torigoe T Homma K Takahashi S Cruikshank W W Jimbow K Sato N |
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Institution: | Division of Plastic Surgery, Sapporo Medical University School of Medicine, Sapporo, 060-8543, Japan. tfujita@sapmed.ac.jp |
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Abstract: | It is well known that it is difficult to induce an immunotolerance with allogeneic skin transplantation. We attempted to find the immunosuppressive protocol for prolonging skin allograft rejection by using interleukin-16 because IL-16 is considered one of the natural ligands to CD4 molecules. First we examined whether synergistic immunosuppressive effects of recombinant IL-16 plus anti-CD4 mAbs are induced in mixed lymphocyte reaction (MLR). Next we used IL-16-cDNA-transfected OSC-20 (human oral squamous cell carcinoma cell line) as an in vitro model of the epidermal keratinocyte equivalent and examined whether this transfectant could inhibit the activation of allogeneic T cells. Our data indicated that IL-16 clearly inhibited human MLR and that IL-16 increased synergistically the immunosuppressive effect of anti-CD4 mAb. We also used IL-16 transfectant and this produced more than 50 ng/ml of IL-16 in the supernatant by which human MLR was significantly inhibited. Furthermore, this transfectant also inhibited the activation of allogeneic lymphocytes stimulated directly with transfectant cells. These results indicated that the IL-16-producing allogeneic skin graft might have a local immunosuppressive action that would prolong graft survival. |
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