Carbon-13 nuclear magnetic resonance study of spin labelled cholesteryl ester in model membranes |
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Authors: | WDale Treleaven Stephen R Wassall Robert J Cushley |
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Institution: | Department of Chemistry, Simon Fraser University, Burnaby, British Columbia. V5A 1S6 Canada |
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Abstract: | Carbon-13 NMR longitudinal relaxation times for unilamellar vesicles of egg phosphatidyl-choline (PC) in aqueous dispersion have been measured following the incorporation of spin labelled cholesteryl palmitate. The spin label induced relaxation rates. 1/T1.5L, for fatty acyl chain carbons show that the C5 segment of the cholesteryl ester acyl chain is located near the C1 and C2 segments of the phospholipid acyl chains. A greater spin label induced enhancement of relaxation rate was observed for the inner vesicle layer than for the outer, and is attributed to a higher ester incorporation and/or tighter lipid packing in the inner layer. |
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Keywords: | carbon-13 longitudinal relaxation egg phosphatidylcholine vesicles 5-doxyl cholesteryl palmitate cholesteryl ester position |
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