A Pumpkin 72-kDa Membrane Protein of Precursor-Accumulating Vesicles Has Characteristics of a Vacuolar Sorting Receptor |
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Authors: | Shimada Tomoo; Kuroyanagi Miwa; Nishimura Mikio; Hara-Nishimura Ikuko |
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Institution: | 1 Department of Cell Biology, National Institute for Basic Biology Okazaki, 444 Japan
2 Department of Molecular Biomechanics, School of Life Science, The Graduate University for Advanced Studies Okazaki, 444 Japan |
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Abstract: | Precursor-accumulating (PAC) vesicles were previously shownto mediate the transport of the precursor of a major storageprotein (pro2S albumin) to protein-storage vacu-oles in developingpumpkin cotyledons. In this study, we characterized two homologousproteins from PAC vesicles, a 72 kDa protein (PV72) and an 82kDa protein (PV82). PV72 and PV82 showed an ability to bindto peptides derived from both an internal propeptide and a C-terminalpeptide of pro2S albumin. PV72 was predicted to be a type Iintegral membrane protein with epidermal growth factor (EGF)-likemotifs. These results suggest that PV72 and PV82 are potentialsorting receptors for 2S albumin to protein-storage vacuoles. (Received August 25, 1997; Accepted October 17, 1997) |
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