Crystallization of a DNA tridecamer d(C-G-C-A-G-A-A-T-T-C-G-C-G) |
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| Authors: | M A Saper H Eldar K Mizuuchi J Nickol E Appella J L Sussman |
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| Affiliation: | 1. Department of Structural Chemistry The Weizmann Institute of Science Rehovot 76100, Israel;2. Laboratory of Molecular Biology National Institute of Arthritis, Diabetes and Digestive and Kidney Diseases National Institutes of Health Bethesda, MD 20205, U.S.A.;3. National Cancer Institute National Institutes of Health Bethesda, MD 20205, U.S.A.;1. Histology and Cell Biology Department, Faculty of Medicine, Zagazig University, Egypt;2. Forensic Medicine and Clinical Toxicology Department, Faculty of Medicine, Zagazig University, Egypt;1. Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Baldiri Reixac 10–12, Barcelona 08028, Spain;2. Joint BSC-IRB Research Program in Computational Biology, Baldiri Reixac 10–12, Barcelona 08028, Spain;3. Department of Biochemistry and Molecular Biology, University of Barcelona, Barcelona 08028, Spain |
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| Abstract: | Crystals of the DNA tridecamer d(C-G-C-A-G-A-A-T-T-C-G-C-G) have been grown by the vapor-diffusion technique with 2-methyl-2,4-pentanediol as precipitant. They are monoclinic space group C2, with a = 79.6 A, b = 43.1 A, c = 24.9 A and beta = 98.7 degrees. Previous nuclear magnetic resonance studies predicted that this tridecamer forms a duplex similar to the B DNA dodecamer, d(C-G-C-G-A-A-T-T-C-G-C-G), except for an extra adenosine residue that is stacked within the helix but remains unpaired: (formula; see text) Preliminary X-ray diffraction studies confirmed that the tridecamer is in the B DNA conformation, consistent with the nuclear magnetic resonance results. |
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