Ultrastructural characterization of two new human endometrial carcinoma cell lines and normal human endometrial epithelial cells cultured on extracellular matrix |
| |
Authors: | Jeff A. Boyd Clifford A. Rinehart Jr. Leslie A. Walton Gene P. Siegal David G. Kaufman |
| |
Affiliation: | (1) Department of Obstetrics and Gynecology, University of North Carolina at Chapel Hill, 27599 Chapel Hill, North Carolina;(2) Lineberger Cancer Research Center, University of North Carolina at Chapel Hill, 27599 Chapel Hill, North Carolina;(3) Present address: Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, P.O. Box 12233, 27709 Research Triangle Park, NC;(4) Department of Pathology, University of North Carolina, Campus Box 7525, 27599 Chapel Hill, NC |
| |
Abstract: | Summary Two new lines of human endometrial carcinoma (HEC) cells, one from an adenocarcinoma and one from a highly metastatic serous papillary carcinoma, were established in culture. Structural and morphologic properties of these cells at early passage were compared with those of cultured normal human endometrial epithelial (NHEE) cells. For these studies, cells were grown on a conventional plastic surface or on an extracellular matrix substrate (Matrigel), and examined by transmission electron microscopy and immunofluorescent light microscopy. The HEC cells appeared morphologically similar on plastic and Matrigel, whereas the NHEE cells showed significantly greater epithelial morphologic differentiation on Matrigel than on plastic. On extracellular matrix, the morphologic differences observed between HEC cells and NHEE cells were primarily of an architectural nature, which may be in part explained by differences between NHEE and HEC cells in the arrangement of actin microfilaments and cytokeratin intermediate filaments. Furthermore, HEC cells displayed extensive networks of vimentin intermediate filaments, which were absent from the NHEE cells. These observations support the hypothesis that architectural deregulation is a prominent feature of endometrial carcinoma, and that cytoskeletal alterations may uncouple HEC cell ultrastructural morphology from the influence of extracellular matrix. This research was supported by research grants CA31733, CA45727, and ES07017, from the National Institutes of Health, Bethesda, MD. G. P. S. is a Jefferson Pilot Fellow in Academic Medicine. A preliminary account of this work was presented at the 1988 U.S.-Canadian Academy of Pathology Annual Meeting (Lab. Inves. 58:12a, 1988). |
| |
Keywords: | endometrium epithelial carcinoma ultrastructural extracellular matrix |
本文献已被 SpringerLink 等数据库收录! |
|