Aerobic microbial degradation of alginate in Laminaria hyperborea stipes containing different levels of polyphenols |
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Authors: | Einar Moen Bjørn Larsen Kjetill Østgaard |
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Institution: | (1) Norwegian Biopolymer Laboratory and Department of Biotechnology, The Norwegian University of Science and Technology, N-7034 Trondheim, Norway |
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Abstract: | The polyphenols present in brown seaweed tissue may seriously affect aerobic microbial degradation, particularly the alginate
present. Laminaria hyperborea stipes, harvested at 59 °N off the Norwegian coast in autumn, were degraded at different levels
of polyphenols in aerated batch reactors at 35 °C and pH 7. This was achieved by manipulating the relative amounts of peripheral
tissue, by removing or adding the mechanically peeled outer phenolic layer, using standardized inocula already adapted to
L. hyperborea degradation. The degradation of organic matter was clearly depressed by increasing the amount of peripheral
tissue. Alginate lyase activity was also negatively correlated to the amount of peripheral tissue loaded, presumably due to
the release of reactive polyphenols. The total digestion rates of alginate were reduced by more than a factor of two at enhanced
amounts of peripheral tissue. The guluronic content of extracted Na-alginate increased during the degradation, despite the
presence of significant amounts of guluronate specific alginate lyase activity.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | seaweed aerobic degradation Laminaria hyperborea alginate lyase polyphenols |
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