Dominance of Lactobacillus plantarum strains in grass silage as demonstrated by a novel competition assay |
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Authors: | Fiona Duffner A Fitzsimons G Brophy P O'Kiely M O'Connell |
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Institution: | School of Biological Sciences, Dublin City University, Dublin;Teagasc, Grange Research Centre, Dunsany, County Meath, Ireland |
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Abstract: | An assay was developed for assessing the competitive ability of potential Lactobacillus plantarum silage inoculants. This assay was based on the ability of the test inoculant to outcompete a standard strain ( Lact. plantarum DCU101) co-inoculated at the same rate of 5 × 105 colony forming units g-1 of grass. Total populations of Lact. plantarum were enumerated with a selective medium and Lact. plantarum DCU101 was identified with a strain-specific DNA probe. The DNA probe was based on a small (2.2 kb), cryptic, indigenous plasmid which was cloned into pAT153, a multicopy cloning vector. Seven Lact. plantarum strains, six of which were isolated from well-preserved grass silages, were used to inoculate laboratory scale silos, and variation in strain dominance was monitored over the 14 d ensilage period. |
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