The influence of carbon sources and mononucleotides on the production of extracellular alkaline phosphatase by bacillus intermedium |
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Authors: | M R Sharipova N P Balaban N V Nekhotyaeva I B Leshchinskaya |
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Institution: | (1) Kazan State University, ul. Kremlevskaya 18, 420008 Kazan, Tatarstan, Russia |
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Abstract: | The biosynthesis of extracellular alkaline phosphatase in the streptomycin-resistant strainsBacillus intermedius S3-19 and S7 in the presence in the medium of 5’-nucleoside monophosphates and different sources of carbon—glucose, sodium
pyruvate, sodium lactate, or glycerol—was studied. It was established that, in the presence of mononucleotides, the content
of extracellular alkaline phosphatase in both strains increased; the maximal effect was caused by 5’-AMP at a concentration
of 20μg/ml. In medium with a low orthophosphate content, where active biosynthesis of alkaline phosphatase occurred, 1% glucose and 0.5%
pyruvate stimulated this process 2.5–4 times, and 2% sodium lactate and sodium pyruvate, on the contrary, inhibited it by
20–40%. Analysis of the dynamics of growth and accumulation of extracellular phosphatase in the presence of different sources
of carbon in the medium gives evidence of an interrelationship between the biosynthesis of alkaline phosphatase and carbon
metabolism inBacillus intermedius. |
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Keywords: | extracellular alkaline phosphatase ofBacillus intermedius biosynthesis mononucleotides sources of carbon |
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