A secondary attachment site for bacteriophage lambda in trpC of E. coli. |
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| Authors: | G E Christie T Platt |
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| Affiliation: | Department of Molecular Biophysics and Biochemistry Yale University 333 Cedar Street New Haven, Connecticut 06510 USA |
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| Abstract: | We have determined the nucleotide sequence of a secondary lambda attachment site in trpC. Direct sequence analysis of lambdatrp transducing phage DNA fragments carrying the two prophage attachment sites reveals a 6 nucleotide homology in the crossover region which is a subset of the 15 nucleotide core sequence in the primary lambda attachment site: GCTTTTTTATACTAA. This 6 nucleotide sequence is also present in the intact trpC genome at the attachment site, as shown by analysis of trpC mRNA spanning this region. |
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| Keywords: | To whom requests for reprints should be addressed. |
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