Monitoring immediate-early gene expression through firefly luciferase imaging of HRS/J hairless mice |
| |
Authors: | Anne?M?Collaco Email author" target="_blank">Michael?E?GeuszEmail author |
| |
Institution: | (1) Department of Biological Sciences and J. P. Scott Center for Neuroscience, Behavior and Mind, 217 Life Science Building, Bowling Green State University, Bowling Green, OH 43403-0212, USA |
| |
Abstract: | Background Gene promoters fused to the firefly luciferase gene (luc) are useful for examining gene regulation in live transgenic mice and they provide unique views of functioning organs. The
dynamics of gene expression in cells and tissues expressing luciferase can be observed by imaging this enzyme's bioluminescent
oxidation of luciferin. Neural pathways involved in specific behaviors have been identified by localizing expression of immediate-early
genes such as c-fos. A transgenic mouse line with luc controlled by the human c-fos promoter (fos::luc) has enabled gene expression imaging in brain slice cultures. To optimize imaging of immediate-early gene
expression throughout intact mice, the present study examined fos::luc mice and a second transgenic mouse containing luc controlled by the human cytomegalovirus immediate-early gene 1 promoter and enhancer (CMV::luc). Because skin pigments and
hair can significantly scatter light from underlying structures, the two transgenic lines were crossed with a hairless albino
mouse (HRS/J) to explore which deep structures could be imaged. Furthermore, live anesthetized mice were compared with overdosed
mice. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|