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Callus formation in Ecklonia cava Kjellman (Laminariales,Phaeophyta) |
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| Authors: | Kawashima Yukio Tokuda Hiroshi |
| Affiliation: | (1) Japan NUS Co., Ltd, Environmental Service Division, Daiichi Seimei Bldg. 16F, Nishi-shinjuku 2-7-1, 163 Shinjuku-ku, Tokyo, Japan;(2) Department of Biotechnology, Graduate School of Agriculture Science, University of Tokyo, Yayoi 1-1-1, 113 Bunkyo-ku, Tokyo, Japan |
| Abstract: | Explants from stipes and meristems of Ecklonia cava were incubated on six media under several culture conditions. Both stipe and meristem explants developed calluses three to six weeks after inoculation onto all media except AS PC-1. Calluses developed on stipe explants but did not develop on meristem explants at a temperature of 23 °C. Temperatures from 8 to 13 °C were favorable for callus development. Callus development on meristem explants required light but callus development on stipe explants did not. |
| Keywords: | callus Ecklonia cava Laminariales Phaeophyta tissue culture |
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