Highly efficient over-production in E. coli of YvcC, a multidrug-like ATP-binding cassette transporter from Bacillus subtilis |
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| Authors: | Emmanuelle SteinfelsCé dric Orelle,Olivier DalmasFranç ois Penin,Bruno MirouxAttilio Di Pietro,Jean-Michel Jault |
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| Affiliation: | a Institut de Biologie et Chimie des Protéines, UMR 5086 CNRS/Université Claude Bernard Lyon I, 7 passage du Vercors, 69367, Lyon, France
b CEREMOD, UPR 9078 CNRS, 9 rue J. Hetzel, 92190, Meudon, France |
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| Abstract: | ATP-binding cassette (ABC) transporters have often been refractory to over-expression. Using the C41(DE3) E. coli as a host strain, membrane vesicles highly enriched (>50%) in YvcC, a previously uncharacterized ABC transporter from Bacillus subtilis homologous to P-glycoprotein multidrug transporters, were obtained. The functionality of YvcC was assessed by its high vanadate-sensitive ATPase activity and its ability to transport a fluorescent drug, the Hoechst 33342. |
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| Keywords: | YvcC ATP-binding cassette Bacillus subtilis |
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