Modulation of the expression of GABAA receptors in rat cerebellar granule cells by protein tyrosine kinases and protein kinase C |
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Authors: | Raffaella BalduzziAroldo Cupello Mauro Robello |
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Institution: | a Unità INFM, Dipartimento di Fisica, Università di Genova, Via Dodecaneso 33, 16146 Genoa, Italy b Centro di Neurofisiologia Cerebrale, C.N.R., Genoa, Italy |
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Abstract: | The expression of GABAA receptors in rat cerebellar granules in culture has been studied by β2/3 subunit immunocytochemistry and fluorescence confocal microscopy. These cells show labeling all over the cell bodies' plasma membrane and dendrites. Treatment with the protein tyrosine kinase (PTK) inhibitor genistein results in a decrease of the labeling associated with the β2/3 subunit in both cell bodies and dendrites. No effect was found with an inactive genistein analogue, daidzein. A similar effect was found with a protein kinase C (PKC) activator, phorbol myristate acetate (PMA). The effects of genistein and PMA are additive.The interpretation of the results is that PTK inhibition blocks exocytotic deposit of newly synthesized GABAA receptors onto the neuronal plasma membrane. On the other hand, PKC activation speeds up endocytotic removal of GABAA receptors. |
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Keywords: | GABAA receptor Granule cell Immunocytochemistry Protein tyrosine kinase Protein kinase C |
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