Towards high-resolution H-NMR in biological membranes: magic angle spinning of bicelles |
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Authors: | Claire CarlottiFabien Aussenac Erick J. Dufourc |
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Affiliation: | Institut Européen de Chimie et Biologie, Ecole Polytechnique, Av. Pey Berland, BP 108, 33607 Pessac cedex France |
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Abstract: | Proton line narrowing in biomembranes spun at the magic angle, for spinning speeds greater than 7 kHz, was investigated in two ways: increasing the field strength from 200 to 800 MHz and changing the membrane fluidity. The resolution that one can obtain on natural lipid membranes under the form of liposomes is 0.019 ppm at 800 MHz. On the other hand, spinning bicelles (disk-like model membranes made of synthetic long and short chain lipids) at the magic angle decreases the line width by an additional factor of 3 provided the bicelle is subjected to large orientational disorder. This leads to proton line widths of the order of 6 Hz at 500 MHz. The conjunction of high field, magic angle spinning and use of bicelle membranes should prove to be useful to solve membrane protein structure in a membrane environment. |
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Keywords: | Bicelle MAS NMR Proton line width in membrane Membrane protein |
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