Involvement of<Emphasis Type="Italic">Leishmania donovani</Emphasis> major surface glycoprotein gp63 in promastigote multiplication |
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Authors: | Sanjeev?Pandey Phuljhuri?Chakraborti Rakhi?Sharma Santu?Bandyopadhyay Dwijen?Sarkar Email author" target="_blank">Samit?AdhyaEmail author |
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Institution: | (1) Indian Institute of Chemical Biology, 4 Raja SC Mullick Road, 700 032 Kolkata, India;(2) Present address: BB College, 713 303 Asansol, Dist., Burdwan, Asansol, India |
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Abstract: | The major surface glycoprotein gp63 of the kinetoplastid protozoal parasiteLeishmania is implicated as a ligand mediating uptake of the parasite into, and survival within, the host macrophage. By expressing
gp63 antisense RNA from an episomal vector inL. donovani promastigotes, gp63-deficient transfectants were obtained. Reduction of the gp63 level resulted in increased generation times,
altered cell morphology, accumulation of cells in the G2/M phase of the cell cycle, and increased numbers of binucleate cells
with one or two kinetoplasts. Growth was stimulated, and the number of binucleate cells reduced, by addition to the culture
of a bacterially expressed fusion protein containing the fibronectin-like SRYD motif and the zinc-binding (metalloprotease)
domain of gp63. These observations support an additional role of gp63 in promastigote multiplication; the fibronectin-like
properties of gp63 may be important in this process |
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Keywords: | Antisense cytokinesis gp63 Leishmania |
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