Extracellular polysaccharides of cowpea rhizobia: compositional and functional studies |
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| Authors: | Bina Mody Meena Bindra Vinod Modi |
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| Affiliation: | (1) Department of Microbiology, Faculty of Science, M.S. University of Baroda, Baroda, India;(2) Biotechnology Centre, Faculty of Science, M.S. University of Baroda, Baroda, India |
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| Abstract: | Polysaccharides excreted by cowpea Rhizobium strains JLn(c) and RA-1 were mixtures of complex acidic exopolysaccharides and low molecular weight neutral glucans. These polymers were fractionated using gel filtration chromatography. Purified fractions of the acidic heteropolymer reacted with peanut agglutinin to give precipitin bands when subjected to Ouchterlony gel diffusion. The acidic exopolysaccharide was found to contain mainly glucose, galactose, glucuronic acid, mannose and fucose. The non-carbohydrate substituents of the acidic heteropolymer were pyruvate, acetate and uronate which were identified by infrared and proton nuclear magnetic resonance spectroscopy as well as by chemical analysis.Abbreviations EPS Extracellular polysaccharide - YEM yeast extract mannitol - PNA peanut agglutination - 1H-NMR proton nuclear magnetic resonance |
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| Keywords: | Cowpea rhizobia Acidic polysaccharide Peanut agglutinin Gel filtration IR and NMR spectroscopy Pyruvate Acetate |
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