An ultrastructural investigation on the polyethylene glycol-induced adhesion of tobacco nuclei and uptake of micronuclei by soybean protoplasts |
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Authors: | B Rathinasabapathi John King |
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Institution: | (1) Department of Biology, University of Saskatchewan, S7N 0W0 Saskatoon, Saskatchewan, Canada;(2) Present address: Institut de Recherche en Biologie Végétale, 4101 rue Sherbrooke Est, PQ H1X 2B2 Montréal, Québéc, Canada |
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Abstract: | Nuclei isolated from tobacco protoplasts were induced to be taken up by soybean protoplasts using a protocol involving polyethylene glycol (PEG), osmotic shock and pH shift. Transmission electron microscopy revealed that PEG treatment condensed the chromatin of the isolated nuclei. Close adhesion of isolated nuclei to the plasma membrane of protoplasts following PEG treatment, was observed by both scanning and transmission electron microscopic methods. Ultrastructural observations were also made on the formation of micronuclei in tobacco cells following the treatment with amiprophosmethyl (APM). Nuclei and micronuclei isolated from APM-treated cells were induced to be taken up by soybean protoplasts. A single case of uptake of an isolated micronucleus was observed by transmission electron microscopy. The observations on the effects of PEG on the isolated nuclei, micronuclei and protoplasts are discussed in relation to the possible mechanism of uptake of nuclei by protoplasts using PEG. |
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Keywords: | amiprophosmethyl isolated nuclei micronuclei organelle uptake polyethylene glycol protoplasts |
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