| 细毛樟愈伤组织培养 |
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| 引用本文： | 周立刚, 程必强, 喻学俭, 王君健, 杨崇仁,. 细毛樟愈伤组织培养[J]. 广西植物, 1998, 18(4): 347-350 |
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| 作者姓名： | 周立刚 程必强 喻学俭 王君健 杨崇仁 |
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| 作者单位： | [1]华中理工大学药物研究所 [2]中国科学院昆明植物研究所 |
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| 基金项目： | 云南省应用基础研究基金 |
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| 摘 要： | 从富含香叶醇的细毛樟（ＣｉｎｎａｍｏｍｕｍｔｅｎｕｉｐｉｌｕｍＫｏｓｔｅｒｍ）叶片外植体诱导出愈伤组织。愈伤组织生长较合适的培养基为ｐＨ５．８附加ＩＡＡ２ｍｇ／Ｌ和ＫＴ０１ｍｇ／Ｌ的ＭＳ培养基，在２５℃下暗培养。愈伤组织无性系继代培养到第６代后芳香成分初步分析结果表明主成分仍为香叶醇。
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| 关 键 词： | 细毛樟；愈伤组织；生长速率；香叶醇 |
Callus culture of Cinnamomum tenuipilum |
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| Zhou Ligang Cheng Biqiang Yu Xuejian Wang Junjian Yang Chongren. Callus culture of Cinnamomum tenuipilum[J]. Guihaia, 1998, 18(4): 347-350 |
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| Authors: | Zhou Ligang Cheng Biqiang Yu Xuejian Wang Junjian Yang Chongren |
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| Affiliation: | Zhou Ligang 1 Cheng Biqiang 2 Yu Xuejian 2 Wang Junjian 1 Yang Chongren 2 |
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| Abstract: | Calli were induced from the leaves explants of a chemical type of Cinnamomum tenuipilum Kosterm which main aromatic component was geraniol. It was optimal for the callus growth by using MS medium in which IAA 2 mg/L and KT 0.1 mg/L were added into it. The optimum pH value in the medium was about 5～6, and optimum temperature was about 25 ℃. Light inhibited callus growth. Preliminary identification of geraniol in the callus was carried out by gas chromatography. The results demonstrated that callus was capable of synthesizing geraniol the same as that of the original plant. |
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| Keywords: | Cinnamomum tenuipilum Kosterm callus growth rate geraniol |
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