The pyridoxal phosphate-binding site of rabbit muscle aldolase |
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Authors: | M Anai C Y Lai B L Horecker |
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Affiliation: | Department of Molecular Biology, Division of Biological Sciences, Albert Einstein College of Medicine, Bronx New York 10461 U.S.A. |
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Abstract: | Under appropriate conditions pyridoxal phosphate forms a Schiff-base derivative with a specific lysine residue in rabbit muscle aldolase, with the incorporation of slightly less than 1 equiv of pyridoxal phosphate per enzyme subunit. Reduction of the Schiff base with tritium-labeled borohydride introduces a radioactive label at this site. A tryptic peptide containing the labeled lysine residue has been isolated and found to possess the following sequence: Gly-Gly-Val-Val-Gly-Ile-Lys1-Val-Asp-Lys, where the asterisk indicates the modified lysine residue. |
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