Effect of interleukin-2 on the expression of cell cycle genes in human T lymphocytes |
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| Authors: | L Kaczmarek B Calabretta R Baserga |
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| Affiliation: | 1. Department of Pathology, Temple University Medical School, 3400 N. Broad Street, Philadelphia, PA 19140 USA;2. Fels Research Institute, Temple University Medical School, 3400 N. Broad Street, Philadelphia, PA 19140 USA;1. School of Food Science and Engineering, Hefei University of Technology, Hefei, Anhui 230009, People’s Republic of China;2. National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian, Liaoning 116034, People’s Republic of China;3. Department of Chemistry Engineering, Xuzhou College of Industrial Technology, Xuzhou, Jiangsu 221140, People’s Republic of China |
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| Abstract: | We have studied the expression of seven cell cycle-dependent genes in phytohemagglutinin (PHA)-stimulated peripheral blood mononuclear cells, in macrophage-depleted cultures and in macrophage-depleted cultures plus Interleukin-2 (IL-2). The expression of all seven genes is increased in PHA stimulated peripheral cells. Only two (2F1 and the IL-2 receptor) are increased in PHA-stimulated macrophage depleted cultures. Addition of IL-2 to these cultures increased the RNA levels of four genes (KC-1, c-myc, beta-actin and IL-2R), but has no effect on three others (4F1, 2F1, and JE-3). The results indicate that the expression of these cell cycle genes is regulated by different components of the mitogenic stimulus. |
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