Optimization of an Aspergillus niger glucose oxidase production process |
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| Authors: | A. Rothberg J. Weegar R. von Schalien K. Fagervik M. Rydström K. Lind |
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| Affiliation: | (1) Department of Chemical Engineering, University of Cape Town, Private Bag, Rondebosch, 7701, South Africa;(2) Electron Microscope Unit, University of Cape Town, Private Bag, Rondebosch, 7701, South Africa;(3) Present address: Department of Process Engineering, University of Stellenbosch, Private Bag X1, Stellenbosch, 7602, South Africa |
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| Abstract: | The purpose of the present study was to ascertain the optimal concentration of dissolved oxygen in order to maximize the intracellular glucose oxidase formation in Aspergillus niger. Cultivations performed in a 3.5 l laboratory reactor showed that a dissolved oxygen concentration at 3% of saturation at a total pressure of 1.2 bar was optimal for maximizing intracellular glucose oxidase activity. Cultivations performed at higher dissolved oxygen concentrations did not produce as much glucose oxidase as those performed at 3%, although the formation rate was high. Experiments revealed that maximal intracellular glucose oxidase formation for the A. niger strain used, is accomplished by limiting the gluconic acid production rate by means of maintaining a low dissolved oxygen concentration. Several attempts to achieve higher intracellular glucose oxidase activity were also made by manipulating the glucose concentration at a 3% dissolved oxygen concentration. However, no enhancement in glucose oxidase activity was observed. |
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