Long-term effects of thidiazuron are intermediate between benzyladenine,kinetin or isopentenyladenine in Miscanthus sinensis |
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Authors: | Jeanette Møller Nielsen Kirsten Brandt Jürgen Hansen |
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Institution: | (1) Department of Plant Breeding and Propagation, Research Center for Horticulture, Danish Institute of Plant and Soil Science, Kirstinebjergvej 10, DK-5792 Årslev, Denmark |
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Abstract: | The thidiazolylurea derivative thidiazuron has been reported to be considerably more effective than benzyladenine in promotion of in vitro shoot formation in a number of dicotyledonous species. In the present study, axillary shoots of Miscanthus sinensis (Thunb.) Anderss. Giganteus that had been subcultured four times on modified Murashige & Skoog medium with 20 M benzyladenine were transferred to media with benzyladenine, kinetin, isopentenyladenine or thidiazuron at concentrations of 0.01, 0.1, 1, 10, 30 or 100 M and grown over four subcultures. Shoot and root formation stabilized after the first subculture and results from the three subsequent subcultures are presented. The common effects of cytokinins, i.e., promotion of axillary bud growth, inhibition of root formation, reduced stem growth and delay of senescence, were observed for all four cytokinins. In a descending order regarding shoot formation, the four cytokinins at the optimum concentration could be ranked as follows: benzyladenine, thidiazuron, kinetin and isopentenyladenine. Benzyladenine and thidiazuron had optimum effects at the same concentration with regard to axillary shoot formation but thidiazuron induced a significantly lower number of shoots than benzyladenine. The number of roots, shoot size and percentage of chlorotic shoots were also the same for benzyladenine and thidiazuron. When transferring shoots from benzyladenine or thidiazuron medium to rooting medium, shoots previously grown on thidiazuron became taller and formed fewer roots than shoots previously grown on benzyladenine.Abbreviations BA
benzyladenine
- 2iP
isopentenyladenine
- KIN
6-(furfurylamino)-purine (kinetin)
- MS
Murashige & Skoog medium
- NAA
naphthaleneacetic acid
- THI
N-phenyl-N(1,2,3-thidiazol-5-yl)-urea (thidiazuron) |
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Keywords: | Axillary shoots cytokinin Poaceae root formation senescence |
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