PCR primer design: an inquiry-based introduction to bioinformatics on the World Wide Web |
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| Affiliation: | 1. College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, QAU, Qingdao 266109, China;2. Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266061, China;1. Smurfit Institute of Genetics, Trinity College Dublin, Dublin 2, Ireland;1. Departamento de Química Inorgánica, Facultad de Química, Universidad Nacional Autónoma de México, México D.F. 04510, Mexico;2. Instituto de Investigaciones en Materiales, Universidad Nacional Autónoma de México, México D.F. 04510, Mexico;3. Departamento de Física y Química Teórica, Facultad de Química, Universidad Nacional Autónoma de México, México D.F. 04510, Mexico;1. Department of Pediatrics, University of Rochester, United States;2. Department of Pathology and Laboratory Medicine, University of Rochester, Rochester, NY, United States |
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| Abstract: | A project is described which details the selection of PCR primers for an unknown gene of the Herpes Simplex Virus (Type 1) using tools available on the World Wide Web. Within the context of designing PCR primers, students are introduced to some of the basic tools used in the field of bioinformatics and have the opportunity to further develop the concepts of nucleic acid sequence and structure, based on the analysis of selected nucleotide primers. |
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