Histone messenger RNAs of the mouse testis |
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| Authors: | R D Faulkner E C Whisenant Y M Bhatnagar |
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| Institution: | 2. Ophthalmology Department, Faculty of Medicine, University of Pristina, Kosovska Mitrovica, Serbia |
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| Abstract: | A 6-12S RNA fraction has been isolated following sucrose gradient fractionation of mouse testis RNA, and further resolved into poly A+ and poly A- RNA fractions by oligo-(dt)-cellulose chromatography. Polyacrylamide gel electrophoresis of products formed in a reticulocyte lysate-dependent cell-free translation system has enabled identification of histone variants, H1t, H2S, H2A . X, an H4-like protein and a low Mr protein (presumably TP and/or protamine). Cell-free synthesis of a number of these histone variants appears to be directed by poly A+ mRNAs. |
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