Role of Escherichia coli DNA polymerase IV in in vivo replication fidelity |
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Authors: | Kuban Wojciech Jonczyk Piotr Gawel Damian Malanowska Karolina Schaaper Roel M Fijalkowska Iwona J |
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Institution: | Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego. 5A, 02106 Warsaw, Poland. |
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Abstract: | We have investigated whether DNA polymerase IV (Pol IV; the dinB gene product) contributes to the error rate of chromosomal DNA replication in Escherichia coli. We compared mutation frequencies in mismatch repair-defective strains that were either dinB positive or dinB deficient, using a series of mutational markers, including lac targets in both orientations on the chromosome. Virtually no contribution of Pol IV to the chromosomal mutation rate was observed. On the other hand, a significant effect of dinB was observed for reversion of a lac allele when the lac gene resided on an F'(pro-lac) episome. |
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