Poly(A)-associated RNA in plants |
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Authors: | Sagher D Edelman M Jakob K M |
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Affiliation: | Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel. |
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Abstract: | The RNA associated with poly(A) sequences from Euglena gracilis and Vicia faba has been isolated by binding to millipore filters and characterized by sedimentation velocity centrifugation and electrophoretic mobility. Poly(A)-associated RNA as isolated in solution was highly aggregated. When denatured, it sedimented as a broad peak with a mean value of 16-18 S. This RNA was shown to be covalently linked to poly(A) sequences which are 150-250 nucleotides long. Our size estimates for plant poly(A) and poly(A)-associated RNA are similar to those obtained for animal cells. |
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