白花蛇舌草豆甾醇对肝癌细胞的体内外抑制作用及对其增殖周期、凋亡的影响

目的:研究白花蛇舌草豆甾醇(stigmasterol from Hedyotis diffusa willd.,SHD)对人肝癌细胞SMMC-7721、BEL-7402的体外抑制作用,对肝癌H22的体内抑制作用及对其增殖周期、凋亡的影响。方法:MTT法评价SHD对人肝癌细胞SMMC-7721、BEL-7402的抑制率变化规律。昆明雄性小鼠60只,随机取10只为正常对照组,余接种H22瘤株,随机分为模型对照组、5-FU阳性对照组(30mg/kg)和高中低剂量SHD给药组(剂量分别为15、30、60mg/kg),腹腔给药10 d后,比较各组瘤重抑制率、H22细胞周期分布、凋亡率。结果:SHD对SMMC-7721、BEL-7402细胞具有体外抑制作用;SHD显著抑制H22肿瘤,增加G0-G1期细胞比例,降低G2/M期细胞比例,促进肿瘤细胞凋亡。结论:SHD在体外、体内均具有抑制肝癌细胞的作用,此作用与阻滞肿瘤细胞增殖周期,促进肿瘤细胞凋亡有关。

Inhibitive effect of Stigmasterol from Hedyotis diffusa willd. On hepatoma cells in vitro & vivo and its influence on transplanted H22 tumor cell's multiplication cycle, apoptosis

Objective:This study was designed to investigate inhibitive effect of Stigmasterol from Hedyotis diffusa willd.(SHD) on human hepatoma cell line SMMC-7721,BEL-7402 in vitro,its antitumor effect on transplanted H22 tumor cells in vivo and its influ- ence on the tumor cell's multiplication cycle,apoptosis.Methods:The cultured SMMC-7721 and BEL-7402 cells were treated with dif- ferent concentrations of SHD.The proliferation of the cells was measured by MTT assay.The growth of transplanted H22 tumor in mice, the changes of its multiplication cycle and apoptosis were investigated.Results:The proliferation of SMMC-7721,BEL-7402 cells in vit- ro was inhibited by SHD.In H22 mice,transplanted tumor was suppressed,the proportion of G0-G1 cells was increased,G2/M cells were reduced,the tumor cell's apoptosis was induced.Conclusion:Hepatoma cells can he inhibited by SHD both in vivo and in vitro.The ef- fects are related with blocking tumor cell multiplication cycle,inducing its apoptosis.