Measurement of the adhesive strength of a gingival cell line to agar |
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| Authors: | Ronald D Fletcher S M Breitling John K Adamiak M L Williams Jr |
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| Institution: | (1) Department of Microbiology, School of Dental Medicine, University of Pittsburgh, 15261 Pittsburgh, Pennsylvania;(2) Department of Metallurgical and Materials Engineering, School of Engineering, University of Pittsburgh, 15261 Pittsburgh, Pennsylvania |
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| Abstract: | Summary A new technique is described for measuring the adhesive strength of a gingival cell line to an agar substratum by the modification
of the original “blister” test for adhesives. A cell monolayer was developed on a Petri dish with a hole in the center of
the growing surface, overlayed with agar, and the system pressurized to debond the cells from the agar surface. Pressure changes
were measured by a capacitance pressure transducer the output of which was measured by a strip-chart recorder. The modulus
(E) of the agar overlay was determined and used in the calculation of the adhesive-bond strength (γa). The (γa) yield for the gingival cell line (cell-agar debond) was 48.8 ergs per cm2, and for the control (no cells) (agar-polystyrene debond) was 30.0 ergs per cm2.
This research was supported by National Institute of Dental Research Grand DE 03983-02 |
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| Keywords: | cell-agar adhesion gingival cell line adhesive strength |
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