Characterization of the yeast ionome: a genome-wide analysis of nutrient mineral and trace element homeostasis in <Emphasis Type="Italic">Saccharomyces cerevisiae</Emphasis> |
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| Authors: | Email author" target="_blank">David?J?EideEmail author Suzanne?Clark T?Murlidharan?Nair Mathias?Gehl Michael?Gribskov Mary?Lou?Guerinot Jeffrey?F?Harper |
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| Institution: | (1) Department of Nutritional Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA;(2) San Diego Supercomputer Center, University of California-San Diego, La Jolla, CA 92903, USA;(3) Biochemistry Department, University of Nevada, Reno, Nevada 89557, USA;(4) Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA |
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| Abstract: | Background Nutrient minerals are essential yet potentially toxic, and homeostatic mechanisms are required to regulate their intracellular
levels. We describe here a genome-wide screen for genes involved in the homeostasis of minerals in Saccharomyces cerevisiae. Using inductively coupled plasma-atomic emission spectroscopy (ICP-AES), we assayed 4,385 mutant strains for the accumulation
of 13 elements (calcium, cobalt, copper, iron, potassium, magnesium, manganese, nickel, phosphorus, selenium, sodium, sulfur,
and zinc). We refer to the resulting accumulation profile as the yeast 'ionome'. |
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