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| 川芎嗪逆转人乳腺癌MCF-7/ADM细胞对阿霉素的耐药性 | |
| 引用本文： | 解霞,吕鹏,高清波,丁艳芳,杨佩满.川芎嗪逆转人乳腺癌MCF-7/ADM细胞对阿霉素的耐药性[J].中国微生态学杂志,2006,18(6):449-450. |
| 作者姓名： | 解霞 吕鹏 高清波 丁艳芳 杨佩满 |
| 作者单位： | 1. 大连大学医学院,解剖学教研室,辽宁,大连,116622 2. 大连医科大学附属二院,实验中心,辽宁,大连,116027 3. 大连医科大学组织胚胎学教研室,辽宁,大连,116027 |
| 摘 要： | 目的 探讨川芎嗪（tetramethylpyrazine,TMP）逆转人乳腺癌MCF-7/ADM细胞对阿霉素（ADM）的耐药性.方法 MTT法测定细胞的药敏性,荧光分光光度法检测细胞内阿霉素浓度的变化,流式细胞术检测耐药细胞凋亡百分率的变化.结果 非细胞毒性剂量（320 mg/L）及低毒剂量（1250 mg/L）川芎嗪均能显著降低MCF-7/ADM的IC50（P＜0.01）,逆转倍数分别为2.13倍和2.82倍;均能显著增加耐药细胞内ADM的浓度（P＜0.01）.320 mg/L川芎嗪能显著增加耐药细胞的凋亡百分率（P＜0.01）.结论 川芎嗪具有部分逆转人乳腺癌MCF-7/ADM细胞对阿霉素的耐药性,其逆转机制与增加细胞内ADM浓度有关. |
| 关 键 词： | 川芎嗪 MCF-7/ADM细胞株 |
| 文章编号： | 1005-376X（2006）06-0449-02 |
| 收稿时间： | 2006-05-23 |
| 修稿时间： | 2006年5月23日 |
Reversal of the resistance to adriamycin in human breast cancer cell line MCF-7/ADM by TMP |
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| XIE Xia,L Peng,GAO Qing-bo,DING Yan-fan,YANG Pei-man.Reversal of the resistance to adriamycin in human breast cancer cell line MCF-7/ADM by TMP[J].Chinese Journal of Microecology,2006,18(6):449-450. | |
| Authors: | XIE Xia L Peng GAO Qing-bo DING Yan-fan YANG Pei-man |
| Institution: | 1. Department of Human Anatomy, Medical College of Dalian University, Dalian 116622, China ; 2. Laborato ry of Molecular Biology ,the Second Hospital of Dalian Medical University, Dalian 116027, China. ; 3. Departrnent of Histology and Embryology, Dalian Medical University, Dalian 116027, China |
| Abstract: | Objective To study the reversal or the resistance to adriamycin in human breast cancer cell line MCF-7/ADM by TMP.Methods The drug sensitivity of MCF-7/ADM cells was measured by MTT assay. The intracellular concentration of ADM was determined by fluorescent-spectrophotometry.The cell apoptosis percentage of MCF-7/ADM was demonstrated by flow cytometry.Results The IC_(50) value of TMP in MCF-7/ADM cell line was significantly decreased by the non-cytotoxic dose(320 mg/L) or low-cytotoxic dose(1250 mg/L) of TMP(P<(0.01)) and the resistance-reversing effect was 2.13-folds, 2.82-folds respectively compared with the IC_(50) value of ADM in MCF-7/ADM cells.The intracellular ADM concentration in MCF-7/ADM cells were significantly elevated by TMP at the same dose(P<0.0l).The cell apoptosis percentage in MCF-7/ADM cells was significantly increased by TMP(P<0.0l).Conclusion TMP could partially reverse the resistance to ADM in human breast cancer MCF-(7/ADM) cell line,which may be related to the increased intracellular ADM concentration. |
| Keywords: | TMP MCF-7/ADM cell line |
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