Studies of the Escherichia coli Rsd-sigma70 complex |
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| Authors: | Westblade Lars F Ilag Leopold L Powell Andrew K Kolb Annie Robinson Carol V Busby Stephen J W |
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| Institution: | School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. westbll@rockefeller.edu |
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| Abstract: | Escherichia coli Rsd protein was previously identified on the basis of its binding to the RNA polymerase sigma(70) subunit. The Rsd-sigma(70) complex has been studied using different methods. Our data show that Rsd associates with sigma(70) to form a complex with a stoichiometry of 1:1. Alanine scanning and deletion mutagenesis were used to locate regions of sigma(70) that are required for the formation of the Rsd-sigma(70) complex. |
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