Determination of remifentanil in human blood by capillary gas chromatography with nitrogen-selective detection |
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Authors: | Bjorksten A R Chan C Crankshaw D P |
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Affiliation: | Anaesthesia Research Laboratory, Department of Anaesthesia and Pain Management, The Royal Melbourne Hospital, Victoria 3050, Australia. |
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Abstract: | A validated method for the determination of remifentanil in human blood, applicable to all therapeutic concentrations, using capillary GC with nitrogen-specific detection and fentanyl as the internal standard has been developed. Citrated whole blood samples were extracted into 1-chlorobutane following precipitation of proteins with methanol. The drugs were back extracted into 10 mM HCl and re-extracted into methanol-1-chlorobutane. The extracts were reconstituted in methanol and injected onto a 25-m BPX-5 column. The lower limit of quantitation was 0.2 ng/ml with within- and between-day coefficients of variation of less than 15%. |
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Keywords: | Remifentanil |
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