| 人生长素基因的合成及克隆 |
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| 引用本文： | 卢希彬,卢步峰,郭礼和,戴平.人生长素基因的合成及克隆[J].生物技术通报,2005(6):69-72. |
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| 作者姓名： | 卢希彬 卢步峰 郭礼和 戴平 |
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| 作者单位： | 1. 华东师范大学,上海,200062
2. 中国科学院上海生命科学研究院生物化学与细胞生物学研究所,上海,200031;上海赛达生物技术研究中心,上海,201203 |
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| 摘 要： | 根据人生长素基因的氨基酸序列,选择大肠杆菌所偏好的密码子,人工设计并合成了20个寡核苷酸片断。通过重叠区扩增法,利用PCR成功地合成了人生长激素基因的全序列。经克隆测序,证明已成功地实现了人生长素基因的合成及克隆。
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| 关 键 词： | 人生长素基因 基因合成 聚合酶链反应 |
| 收稿时间： | 2005-11-01 |
| 修稿时间： | 2005年11月1日 |
Gene Synthesis and Cloning of Human Growth Hormone |
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| Lu Xibin,Lu Bufeng,Guo Lihe,Dai Ping.Gene Synthesis and Cloning of Human Growth Hormone[J].Biotechnology Bulletin,2005(6):69-72. |
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| Authors: | Lu Xibin Lu Bufeng Guo Lihe Dai Ping |
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| Institution: | 1 East China Normal Univestity, Shanghai 200062 ;2 Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031; 3 Shanghai Cel-star Institute of Biotechnology, Shanghai 201203 |
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| Abstract: | According to the amino acids and by selecting high-usage codons in E.coli,we designed and synthesized 20 oligonucleitides DNA fragments with the length about 55bp.By overlap extension PCR method,we successfully synthesized the overall sequence of human growth hormone.The clones of interest were identified by DNA sequencing.The result showed that we have successfully accomplished the synthesis and cloning of human growth hormone. |
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| Keywords: | Human growth hormone gene Gene synthesis Polymerase chain reaction |
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