The effect of 1-deoxymannojirimycin on rat liver alpha-mannosidases |
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| Authors: | J Bischoff R Kornfeld |
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| Institution: | 1. Institut Pasteur, Department of molecular biology & LA CNRS 270, 75724 Paris Cedex 15, France;2. Institut Pasteur, Department of Virology & ER CNRS 147, 75724 Paris Cedex 15, France;3. Centre René Huguenin, 92211 Saint-Cloud, France;4. Institut Curie, Medical Section, 75231 Paris Cedex 05, France |
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| Abstract: | Cloned murine mammary tumor virus (MuMTV) sequences allowed us to search for murine mammary tumor virus related sequences in the DNA of surgically removed human breast tumors. Out of 28 tumors so far examined two were found to contain an Eco RI DNA fragment homologous to the long terminal repeat-group antigen (LTR-Gag) and the Envelope (Env) sequences of MuMTV. We have taken the lymphocytes of these patients and cultured them. Rapid growth of lymphocytes, mostly of T origin, occurred in the presence of T cell growth factor (TCGF). Whereas DNA extracted from fresh lymphocytes is negative, that extracted from the 3-day cultured lymphocytes showed MuMTV related sequences. Long term cultures of T cells and a similar culture derived from a healthy person donor were negative at all stages. DNA extracted from the Ebstein Barr Virus-transformed B cells of the patient does not contain the MuMTV related sequences. |
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| Keywords: | MuMTV Murine Mammary Tumor Virus TCGF T Cell Growth Factor LTR-Gag Long Terminal Repeat - Group antigen EBV Ebstein Barr Virus |
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