Indolepyruvate Pathway for Indole-3-Acetic Acid Biosynthesis in Bradyrhizobium elkanii |
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Authors: | Minamisawa, Kiwamu Ogawa, Ken-Ichiro Fukuhara, Hideyuki Koga, Jinichiro |
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Affiliation: | 1School of Agriculture, Ibaraki University Ami, Ibaraki, 300-03 Japan 2Center for Genetic Ecology, Tohoku University Sendai, Miyagi, 980 Japan 3Bio Science Laboratories, Meiji Seika Kaisha, Ltd. 5-3-1, Chiyoda, Sakado-shi, Saitama, 350-02 Japan |
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Abstract: | Indole-3-acetaldehyde (IAAId) was detected in the culture supernatantof Bradyrhizobium elkanii. Deuteriumlabelled L-tryptophan (Trp)was incorporated into IAAId and indole-3-acetic acid (IAA),suggesting that B. elkanii produces IAA via IAAId from Trp.In B. elkanii cell suspension, indole-3-pyruvic acid (IPyA)was converted to IAAId, and exogenously added IAAId was rapidlyconverted to IAA. Furthermore, the activity of indolepyruvatedecarboxylase (IPDC), which catalyzes the decarboxylation ofIPyA to produce IAAId and is a key enzyme for IPyA pathway,was detected in B. elkanii cell-free extract. The IPDC activitydepended on Mg2+ and thiamine pyrophosphate, cofactors of decarboxylation.This mounting evidence strongly suggests that IAA synthesisoccurs via IPyA pathway (Trp IPyA p IAAId IAA) in B. elkanii. (Received December 11, 1995; Accepted March 4, 1996) |
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