Interest of fed-batch culture for the production of a membrane-bound protein by an adherent animal cell

The production of a membrane-bound protein by culture and harvesting of r-CHO cells has been evaluated. While in batch culture, the maximal cell density is only maintained for a few hours, in a fed-batch one, the cell and enzyme concentrations, as well as the growth and production rates, can be kept constant during 100 hours. This stabilized phase facilitates the cell harvesting and provides a kinetic tool for testing chemical molecules as sodium butyrate.