Hypoxia induces the PDZ domain-containing syntenin in the marine teleost Paralichthys olivaceus |
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Authors: | Hee Jeong Kong Jae-Min Kim Ju-Hyun Moon Young-Ok Kim Bo-Hye Nam Woo-Jin Kim Jeong-Ho Lee Sang-Jun Lee Kyung-Kil Kim Sang-Yeob Yeo Chang Hoon Lee |
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Affiliation: | 1. Biotechnology Research Division, National Fisheries Research and Development Institute, 408-1 Sirang-ri, Gijang-up, Gijang-gun, Busan 619-705, Republic of Korea;2. Genetics and Breeding Research Center, National Fisheries Research and Development Institute, Dapo-ri, Nambu-myon, Geoje, Gyeungnam 656-842, Republic of Korea;3. Department of Biotechnology Division of Applied Chemistry and Biotechnology, Hanbat National University, Daejeon 305-719, Republic of Korea;4. Research Group for Marine and Silver Biotechnology, Pusan National University, 30 Jangjeon-dong, Geumjeong-gu, Busan 609-735, Republic of Korea |
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Abstract: | Syntenin is a scaffolding PDZ domain-containing protein with diverse biological activities, including organization of protein complexes in the plasma membrane, regulation of B-cell development, intracellular trafficking, synaptic transmission, and cancer metastasis. In the present study, we isolated and characterized the cDNA of the olive flounder Paralichthys olivaceus syntenin, designated PoSyntenin. The full-length CDS of PoSyntenin with 5′- and 3′-UTR sequences is 2618 bp long and consists of a 909 bp open reading frame preceded by a 161 bp 5′-UTR and followed by a 1551 bp 3′-UTR. The PoSyntenin cDNA encodes a polypeptide of 302 amino acids containing two PDZ domains, which shares 61–80% homology with those of other species, including humans. Expression of the PoSyntenin mRNA was detectable from 1 day post-hatching and constitutively in the brain, spleen, intestine, stomach, eye, liver, kidney, and gill of normal conditioned fish. Expression of the PoSyntenin mRNA was upregulated in the eye, liver, kidney, spleen, brain, gill, and intestine of flounder under hypoxia and was increased by treatment with the hypoxia-mimic CoCl2 (a HIF-1 inducer) in HINAE cells. Taken together, these results suggest that PoSyntenin is a hypoxia target gene that has a potential role in the hypoxia response mechanism of fish. |
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Keywords: | Syntenin PDZ domain Hypoxia Olive flounder Paralichthys olivaceus |
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