Expression profiles of seven glutathione S-transferase (GST) genes in cadmium-exposed river pufferfish (Takifugu obscurus) |
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| Authors: | Jin-Hyoung Kim Hans-Uwe Dahms Jae-Sung Rhee Young-Mi Lee Jehee Lee Kyung-Nam Han Jae-Seong Lee |
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| Institution: | 1. National Research Lab of Marine Molecular and Environmental Bioscience, Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 133-791, South Korea;2. Department of Marine Science, College of Natural Sciences, Inha University, Incheon 402-751, South Korea;3. Department of Life Science, College of Natural Sciences, Sangmyung University, Seoul 110-743, South Korea;4. Department of Marine Life Science, College of Ocean Science, Jeju National University, Jeju 690-756, South Korea |
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| Abstract: | Glutathione S-transferase (GST; EC 2.5.1.18) plays a critical role in detoxification pathways. In this study, we report cloning and expression of seven genes of the GST family of the pufferfish Takifugu obscurus together with mRNA tissue distribution pattern and time-course of expression in response to exposure to cadmium. At basal levels of tissue expression, GST-Mu is highly expressed in liver compared with other tissues. When fish were exposed to cadmium (5 mg/L for 96 h), expression of GST-MAPEG, GST-Mu, GST-Omega, and GST-Zeta was greatly increased, whereas GST-Alpha and GST-Kappa genes showed no significant response. These findings suggest that gene expression of a number of GST isoforms in T. obscurus is modulated in response to exposure to cadmium. We propose GST-Mu, GST-Theta, and GST-Zeta as candidate biomarkers for heavy metal exposure in this fish. |
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| Keywords: | Takifugu obscurus Antioxidant genes Glutathione S-transferase (GST) Cadmium Detoxification |
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