| 真菌寡糖诱导植物抗性活性成分的分离纯化 |
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| 引用本文： | 石瑛,辛毅,白雪芳,杜昱光.真菌寡糖诱导植物抗性活性成分的分离纯化[J].中国生物化学与分子生物学报,2004,20(1):138-142. |
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| 作者姓名： | 石瑛 辛毅 白雪芳 杜昱光 |
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| 作者单位： | 中国科学院大连化学物理研究所,大连,116023 |
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| 基金项目： | 国家高技术研究发展计划 ( 863计划 )项目 (No .2 0 0 1AA62 0 5 0 1、No .2 0 0 2AA2 45 13 1) |
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| 摘 要： | 寡聚糖作为一种信号分子 ,在调节植物的生长、发育以及植物在不同环境中生存能力等方面起着非常重要的作用1] .许多特定结构的寡糖被证明具有诱导植物抗性的作用 .对具有诱导抗性作用的葡聚寡糖结构分析表明 ,其最小活性寡糖单位是由 7个葡葡糖残基组成的 β 葡聚糖苷 ,在一个
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| 收稿时间： | 2004-02-20 |
| 修稿时间： | 2003年3月10日 |
Isolation and Purification of Oligosaccharide Elicitor from A Fungi Strain |
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| SHI Ying,XIN Yi,BAI Xue-fang,DU Yu-guang.Isolation and Purification of Oligosaccharide Elicitor from A Fungi Strain[J].Chinese Journal of Biochemistry and Molecular Biology,2004,20(1):138-142. |
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| Authors: | SHI Ying XIN Yi BAI Xue-fang DU Yu-guang |
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| Institution: | (Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China |
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| Abstract: | Oligosaccharides derived from cell wall of fungal DL603 were isolated and purified. The hydrolysis of the fungal DL603 with enzyme G603 was conducted, and ion exchange and gel filtration chromatography were exploited for purification of the products. Biologically active fractions were determined by soybean cotyledon assay. Dionex high performance anion exchange chromatography (HPAEC) analysis showed that the oligosaccharides were purified with certain degree of polymerization, and the highest active fractions consisted of glucosamine, galactose, glucose and mannose. |
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| Keywords: | oligosaccharide induced resistance active compound isolation and purification |
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