| 小鼠生精周期判定方法的改进 |
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| 引用本文： | 赵洁,侯武刚,张远强. 小鼠生精周期判定方法的改进[J]. 中国组织化学与细胞化学杂志, 2006, 15(2): 130-134 |
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| 作者姓名： | 赵洁 侯武刚 张远强 |
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| 作者单位： | 第四军医大学基础部组织学与胚胎学教研室,西安,710032 |
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| 基金项目： | 中国科学院资助项目;军队医学科研项目 |
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| 摘 要： | 目的探讨一种可以在普通HE染色或苏木精复染标本上对精子发生周期进行简单划分的方法。方法应用PAS染色法与普通HE染色法相对照,对比观察小鼠睾丸各级生精上皮在精子发生不同时期的形态学变化特点。结果参照Clermont及Russel等制定的生精上皮时相的判定标准,把小鼠生精上皮分为XⅡ个期。结论通过对比总结,可以在普通HE染色或苏木精复染标本上对精子发生周期进行简单划分,并进一步辨别各型生精细胞。
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| 关 键 词： | 精子发生 精子形成 生精时相 顶体 小鼠 睾丸 |
| 收稿时间： | 2005-04-29 |
| 修稿时间： | 2005-11-03 |
IMPROVED METHOD TO EVALUATE SEMINIFEROUS EPITHELIUM STAGE IN MOUSE TESTIS |
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| Zhao Jie,Hou Wugang,Zhang Yuanqiang. IMPROVED METHOD TO EVALUATE SEMINIFEROUS EPITHELIUM STAGE IN MOUSE TESTIS[J]. Chinese Journal of Histochemistry and Cytochemistry, 2006, 15(2): 130-134 |
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| Authors: | Zhao Jie Hou Wugang Zhang Yuanqiang |
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| Abstract: | Objective To search for a simple method to evaluate the seminiferous epithelium stage in specimens of HE or hematoxylin staining.Methods By using two staining methods-the PAS(Periodic Acid Schiff) technique and the HE technique-we observed the morphological changes in the seminiferous epithelia of specific stages.Results According to the criterion of Clermont and Russell,a cycle of the seminiferous epithelia in mouse could be divided into 12 periods.Conclusion By comparison and summary,in HE staining or hematoxylin staining specimen,it is easy to evaluate the stage of seminiferous epithelia in adult mouse testis and precisely distinguish seminiferous epithelia in HE or hematoxylin-stained specimans. |
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| Keywords: | Spermatogenesis Spermiogenesis Spermatogenic stage Acrosome Mouse Testis |
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