肺炎克雷伯氏菌利用甘油生产1,3-PDO发酵优化的研究进展

发展可再生能源，尤其是生物能源，具有显著的能量收益和碳减排效益。随着石油等不可再生资源的减少，许多大宗传统石油化工产品正不断被使用可再生原料的生物制造产品替代。生物发酵法生产1,3-丙二醇(1,3-PDO)顺应了这一潮流，具有广阔的发展前景。提高微生物发酵竞争力，优化发酵法生产1,3-PDO水平，势必增加1,3-PDO的生产效益。对肺炎克雷伯氏菌(Klebsiella pneumoniae)发酵法进行1,3-PDO生产的代谢机理、菌株筛选和利用、发酵参数的选择和优化以及发酵工程策略的设计和监测等进行综述，为利用生物柴油副产物甘油生产有重要工业价值的1,3-PDO产品提供参考。

Advances in Optimizing Fermentation Conditions to Improve the Production of 1,3-Propanediol by Klebsiella pneumoniae Using Glycerol as Substrate

The development of renewable energy, especially bioenergy, has significant energy and carbon emission reduction benefits. With the reduction of non renewable resources such as petroleum, many staple traditional petrochemical products are constantly replaced by industrial biological manufacturing products from renewable raw materials. Bio-fermentation production of 1,3-propanediol (1,3-PDO) conforms to this trend and has broad development prospects. Improving the competitiveness of microbial fermentation, optimizing the fermentation production level of 1,3-PDO and developing high-efficiency fermentation process are bound to increase the production benefits of 1,3-PDO. In this paper the metabolic mechanism, strain screening and utilization, selection and optimization of fermentation parameters, Klebsiella pneumoniae fermentation engineering strategy, as well as design, test and determination to produce 1,3-PDO are reviewed, so as to provide scientific references for the utilization of biodiesel byproduct production of glycerol to produce product of 1,3-PDO with significantly industrial value.