Specific cytogenetic labeling of bovine spermatozoa bearing X or Y chromosomes using fluorescent in situ hybridization (FISH) |
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| Authors: | Fran?ois Piumi Daniel Vaiman Edmond-Paul Cribiu Bernard Guérin Patrice Humblot |
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| Affiliation: | 1.UNCEIA, Services techniques, Maisons-Alfort, France;2.Département de génétique animale, Laboratoire de génétique biochimique et de cytogénétique, Groupe petits ruminants, Institut national de la recherche agronomique, 78352 Jouy-en-Josas, France;3.Laboratoire de radiobiologie et d''étude du génome, Institut national de la recherche agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France |
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| Abstract: | X and Y specific probes were identified in order to apply the fluorescent in situ hybridization (FISH) technique to bovine spermatozoa. For Y chromosome detection, the BRY4a repetitive probe, covering three quarters of the chromosome, was used. For X chromosome detection, a goat Bacterial Artificial Chromosome (BAC) specific to the X chromosome of bovine and goats and giving a strong FISH signal was used. Each probe labeled roughly 45% of sperm cells. The hybridization method will be useful for evaluating the ratio of X- and Y- bearing spermatozoa in a sperm sample and consequently can be used to evaluate the efficiency of sperm sorting by different techniques such as flow cytometry. |
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| Keywords: | fluorescent in situ hybridization spermatozoon X chromosome Y chromosome bacterial artificial chromosome |
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