Keratinase of an anaerobic thermophilic bacterium <Emphasis Type="Italic">Thermoanaerobacter</Emphasis> sp. Strain 1004-09 isolated from a hot spring in the Baikal rift zone |
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Authors: | I V Kublanov K B Tsiroulnikov E N Kaliberda L D Rumsh T Haertlé E A Bonch-Osmolovskaya |
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Institution: | (1) Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7/2, Moscow, 117312, Russia;(2) L’Institute National de la Recherche Agronomique, Nantes, France;(3) Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia |
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Abstract: | A thermophilic anaerobic bacterial strain 1004-09 belonging to the genus Thermoanaerobacter and capable of growth on protein substrates such as albumin, gelatin, casein, and α- and β-keratins was isolated from the Urinskii hot spring (Barguzin river valley, Republic of Buryatia, Russia). A 150-kDa serine proteinase was revealed in the strain supernatant; it exhibited optimal activity at 60°C and pH 9.3 and was capable of keratin hydrolysis. A number of characteristics for the strain 1004-09 keratinase were established including activation by SDS and NaCl and residual activity (15% to the activity of the intact protein) in the presence of 10% ethanol and acetone. |
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Keywords: | thermophilic microorganisms hydrolytic microorganisms proteinases protein hydrolysis keratin |
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