工业模式微生物膜有序性的活细胞定量分析

作为一种相位敏感的荧光探针,Di-4-ANEPPDHQ可以特异性标记膜的有序相和无序相,在理论上可以对细胞膜的有序性进行定量成像。通过将Di-4-ANEPPDHQ和激光扫描共聚焦显微术相结合,对多种具有代表性的工业模式微生物进行了有序相和无序相活细胞成像,结合极性归一化数值的统计比较,最终实现对上述工业模式微生物细胞膜有序性的定量分析,为细胞膜工程提供了一种直观且快速的活细胞检测方法。

Quantitative Analysis of Membrane Ordering of Living Industrial Model Microorganisms

As a phase sensitive fluorescent probe,Di-4-ANEPPDHQ can specifically label the ordered phase and disordered phase of membrane.Therefore,in theory,the probe can be used to quantitatively image the order of cell membrane.By combining Di-4-ANEPPDHQ and laser scanning confocal microscopy,the ordered phase and disordered phase live-cell imaging of a variety of representative industrial model microorganisms were carried out.Combined with the statistical comparison of polarity normalization values,the quantitative analysis of the cell membrane ordering of the above industrial model microorganisms is finally realized.The above study provides an intuitive and rapid detection method of living cells for cell membrane engineering.